Human blood platelets contract in perpendicular direction to shear flow.

In their physiological environment, blood platelets are permanently exposed to shear forces caused by blood flow. Within this surrounding, they generate contractile forces that eventually lead to a compaction of the blood clot. Here, we present a microfluidic chamber that combines hydrogel-based traction force microscopy with a controlled shear environment, and investigate the force fields platelets generate when exposed to shear flow in a spatio-temporally resolved manner.

Rapid Acquisition of X-Ray Scattering Data from Droplet-Encapsulated Protein Systems.

Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single-phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent the sample from adsorbing to the channel walls. In recent years, small-angle X-ray scattering (SAXS) in combination with microfluidics has evolved as a nanoscale method of such systems.

Following DNA Compaction During the Cell Cycle by X-ray Nanodiffraction.

X-ray imaging of intact biological cells is emerging as a complementary method to visible light or electron microscopy. Owing to the high penetration depth and small wavelength of X-rays, it is possible to resolve subcellular structures at a resolution of a few nanometers. Here, we apply scanning X-ray nanodiffraction in combination with time-lapse bright-field microscopy to nuclei of 3T3 fibroblasts and thus relate the observed structures to specific phases in the cell division cycle.

Weight loss with a modified Mediterranean-type diet using fat modification: a randomized controlled trial.

Background/objectives: There is evidence that Mediterranean diets with a high proportion of olive oil and nuts can be effective for weight management and prevention of cardiovascular disease. It might be difficult for populations with other eating habits to follow such diets. Therefore, a modified Mediterranean-type diet using fat modification through neutral and butter-flavored canola oil, walnuts and walnut oil with two portion-controlled sweet daily snacks was tested in Germany.

Multiple internalization pathways of polyelectrolyte multilayer capsules into mammalian cells.

Polyelectrolyte multilayer (PEM) capsules are carrier vehicles with great potential for biomedical applications. With the future aim of designing biocompatible, effective therapeutic delivery systems (e.g.