Detection of empty HLA class II molecules on cord blood B cells.

Fetal mononuclear cells are increasingly used in transplantation of hematopoietic cells due to a reportedly lower incidence of graft-versus-host disease. Previous studies of immune responses of fetal lymphocytes have indicated a general hyporesponsiveness in response to polyclonal stimulation. Fetal B lymphocytes display many features typical of the resting state such as a low level of HLA class II expression, but a large proportion of cells also carry the activation-associated CD23 antigen.

Characterization of lymphocyte subpopulations in cord blood.

Twenty-seven cord blood samples from healthy newborns were processed according to a "whole blood" flow cytometric analysis. The CD3-positive T cells were a variable subpopulation representing 44.8 +/- 13.

Characterization of lymphocyte subpopulations in cord blood.

27 cord blood samples from healthy newborns were processed according to a "whole blood" flow cytometric analysis. The CD3-positive T cells are characterized by their variability: 44.8 +/- 13.

Longitudinal study of the specific humoral and cellular response to Toxoplasma gondii in a patient with acquired toxoplasmosis.

Specific cellular and humoral response to Toxoplasma were evaluated during a 2 yr follow up in a patient with acquired infection. Antibodies were titrated using conventional serological tests and analyzed by western blot technique. Analysis of the patterns obtained with IgA, IgG and IgM antibodies allowed a clear differentiation between acute and chronic stages of infection.

Impaired in vitro lymphocyte response to toxoplasma antigen in HIV1 infected patients.

The containment of Toxoplasma gondii infection is largely dependent of T cell mediated immunity. In this study, in vitro lymphocyte responsiveness to T. gondii antigen was examined in 59 HIV1 infected individuals and in 58 HIV non-infected controls.