Supercontinuum intrinsic fluorescence imaging heralds free view of living systems.

Optimal imaging strategies remain underdeveloped to maximize information for fluorescence microscopy while minimizing the harm to fragile living systems. Taking hint from the supercontinuum generation in ultrafast laser physics, we generated supercontinuum fluorescence from untreated unlabeled live samples before nonlinear photodamage onset. Our imaging achieved high-content cell phenotyping and tissue histology, identified bovine embryo polarization, quantified aging-related stress across cell types and species, demystified embryogenesis before and after implantation, sensed drug cytotoxicity in real-time, scanned brain area for targeted patching, optimized machine learning to track small moving organisms, induced two-photon phototropism of leaf chloroplasts under two-photon photosynthesis, unraveled microscopic origin of autumn colors, and interrogated intestinal microbiome.

Institutional Experience with Curricular Renewal during the OIE Veterinary Education Twinning Program between the University of Peradeniya, Sri Lanka, and Massey University, New Zealand.

The Veterinary Education Twinning Program between the University of Peradeniya (UP) and Massey University (MU) was carried out within the mandate of the World Organisation for Animal Health (OIE) to create opportunities for developing countries to establish educational facilities and methods based on current and accepted international standards. This article describes how the twinning partnership between UP and MU enabled a strong flow of expertise that benefited Sri Lanka to develop a new veterinary undergraduate curriculum. The new curriculum was created to improve the relevance and quality of veterinary education, incorporating current international best practices, to strengthen national veterinary services.

Massive dysregulation of genes involved in cell signaling and placental development in cloned cattle conceptus and maternal endometrium.

A major unresolved issue in the cloning of mammals by somatic cell nuclear transfer (SCNT) is the mechanism by which the process fails after embryos are transferred to the uterus of recipients before or during the implantation window. We investigated this problem by using RNA sequencing (RNA-seq) to compare the transcriptomes in cattle conceptuses produced by SCNT and artificial insemination (AI) at day (d) 18 (preimplantation) and d 34 (postimplantation) of gestation. In addition, endometrium was profiled to identify the communication pathways that might be affected by the presence of a cloned conceptus, ultimately leading to mortality before or during the implantation window.

Subjective Perceived Motor Improvement after Acute Levodopa Challenge in Parkinson's Disease.

Background: Previous studies found a poor association between parkinsonian patient's reported subjective improvement after commencing dopaminergic treatment and improvements in objective measures of motor impairment by clinician.

Objective: To compare PD patient's subjective perceived motor improvement after acute levodopa challenge test with objective motor improvement assessed by the clinician using the UPDRS part III. To analyze clinical characteristics, i.