Publications by authors named "C R Zanetti"
Article Synopsis
- Fibrinolysis plays a crucial role in the release of hematopoietic stem cells from bone marrow, affecting the development of B-cell acute lymphoblastic leukemia (B-ALL).
- Activation of plasmin, driven by annexin A2, alters the extracellular matrix (ECM), which impacts cancer cell growth by trapping the growth factor IGF1 and hindering signaling pathways.
- Inhibiting plasmin activation with ε-aminocaproic acid (EACA) shows promise in reducing tumor size and extending survival in B-ALL models, suggesting that targeting fibrinolysis could be a helpful addition to cancer treatment.
This essay proposes that the only publication of the Accademia del Cimento, referred to as ,1 Accademia del Cimento, (Firenze: Per Giuseppe Cocchini, 1667). had as one of its main goals the celebration of the House of Medici's paternity of cutting-edge experiments and instruments during the reign of Grand Duke Ferdinando II. These included Ferdinando II's thermometers and hygrometers, Torricelli's experiment and barometer, and Galileo's pendulum as a clock-regulator.
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- Eosinophils, when activated by the alarmin IL-33, produce extracellular vesicles (EV) that show potential anti-tumor effects, contrasting with those activated by IL-5.
- Incorporating these IL-33-activated eosinophil-derived EV (Eo33-EV) into tumor cells leads to increased expression of genes that promote cell cycle arrest and reduces tumor growth and metastasis.
- RNA sequencing highlights that Eo33-EV are enriched with tumor suppressor genes and pathways that enhance an epithelial phenotype, indicating their potential role in cancer therapy through cell reprogramming.
Inflammation promotes solid tumor progression, but how regulatory mechanisms of inflammation may affect leukemia is less well studied. Using annexin A5 (ANXA5), a calcium-binding protein known for apoptosis, which we discovered to be differentially expressed in the bone marrow microenvironment (BMM) of mice with acute myeloid (AML) vs chronic myeloid leukemia, as a model system, we unravel here a circuit in which AML-derived tumor necrosis factor α (TNF-α) dose-dependently reduces ANXA5 in the BMM. This creates an inflammatory BMM via elevated levels of prostaglandin E2 (PGE2).
View Article and Find Full Text PDFRespirometric microbial assays are gaining popularity, but their uptake is limited by the availability of optimal O sensing materials and the challenge of validating assays with complex real samples. We conducted a comparative evaluation of four different O-sensing probes based on Pt-porphyrin phosphors in respirometric bacterial assays performed on standard time-resolved fluorescence reader. The macromolecular MitoXpress, nanoparticle NanO2 and small molecule PtGlc and PtPEG probes were assessed with cells in five growth media: nutrient broth (NB), McConkey (MC), Rapid Coliform ChromoSelect (RCC), M-Lauryl lauryl sulfate (MLS), and Minerals-Modified Glutamate (MMG) media.
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