Premise: Despite the high functional importance of endophytes, we still have limited understanding of the biotic and abiotic factors that influence colonization of plant hosts along major ecological gradients and lack quantitative estimates of their colonization extent. In this study, we hypothesized that the developmental stage of the ecosystem will affect the levels of bacterial and fungal endophytic assemblages in the foliar endosphere.
Methods: We quantified levels of bacterial and fungal endophytes in leaves of four plant hosts at four stages of vegetation succession using an optimized qPCR protocol with bacteria-specific 16S and fungi-targeting primers.
Companions' animals can present a risk for the transmission of opportunistic diseases to their owners, including those caused by yeasts of the Candida genus residing in their oral microbiota. This study aimed to isolate and identify yeasts from the oral cavity of dogs and assess their susceptibility to antifungals. Yeast species were identified using automated methods MALDI-TOF-MS and VITEK 2 from 50 dogs (aged 2-4 years, various breeds).
View Article and Find Full Text PDFObjective: To estimate the prevalence and factors associated with the performance of specific admission nursing consultations for SARS-CoV-2 in individuals deprived of liberty with COVID-19 in prison units in Central Brazil.
Method: Analytical cross-sectional study conducted between May and September 2020 through individual interviews and lateral flow immunochromatography tests to detect IgM/IgG antibodies for SARS-CoV-2. The analysis was performed using the Chi-square or Fischer's exact tests, with the outcome being the specific admission nursing consultation for SARS-CoV-2.
Int J Technol Assess Health Care
November 2024
Introduction: Current disease-specific models for prevention of mental disorders are challenged by the overlap of psychopathology, biological mechanisms, and risk factors. Moreover, mental disorders usually begin during childhood or adolescence, when symptoms fluctuate and are highly non-specific.
Discussion: We propose a staging model that integrates three domains - psychopathology, functional impairment and risk factors-, in which prevention is defined as actions to avoid stage progression, irrespective of diagnosis.