[The effect of fatty emulsions with distinct triglyceride compositions on the lipid metabolism of the septic patient].

Objective: To assess the effects on the intravascular lipid mechanism of fatty emulsions with an identical lipid concentration and a different triglyceride composition administered as part of the total parenteral nutrition (TPN) in septicemic patients hospitalized in the intensive care unit (ICU). One emulsion will be made up of long chain triglycerides, LCT (20% Intralipid) (group I) and the other will be made up by a mixture of medium- and long-chain triglycerides, MCT/LCT (1:1) (20% Lipofundina) (group II). AREA: Vall d'Hebrón General University Hospital.

Determination of free serum didanosine by ultrafiltration and high-performance liquid chromatography.

An isocratic reversed-phase high-performance liquid chromatographic method was developed to determine free didanosine concentrations in human serum. An ultrafiltration technique was used to recover didanosine from the samples. didanosine was analyzed using a 150 mm x 3.

Determination of amphotericin B in human serum by liquid chromatography.

A rapid reversed-phase high-performance liquid chromatographic method with a 30-mm long column is described for assaying amphotericin B in serum. After deproteinization of serum samples with methanol, the supernatant was injected onto a reversed-phase C18 column, using 2.5 mM Na2EDTA-acetonitrile (70:30, v/v) as the mobile phase.

Gentamicin pharmacokinetics in adults with bacterial endocarditis.

The pharmacokinetics of gentamicin in adult patients with endocarditis were studied. The records were reviewed for 64 patients treated for bacterial endocarditis and for whom serum gentamicin concentrations had been requested between May 1990 and May 1993. The patients were divided into those with serum creatinine concentration (SCr) <1.

[Results of multicenter evaluation of reagents for determination of sodium, potassium and chloride ions using enzyme activation].

We present the results of a multicentre evaluation with Boehringer Mannheim/Hitachi instruments of new "enzymatic" methods for the determination of Na+, K+, and Cl- in serum or plasma. The between-day coefficient of variation was less than 1.4% (Na+), less than 2.