Monocytic reactive oxygen species-induced T cell apoptosis impairs cellular immune response to SARS-CoV-2 mRNA vaccine.

Background: We have recently shown that, during acute severe COVID-19, SARS-CoV-2 spike protein (S) induces a cascade of events resulting in T cell apoptosis. Indeed, by neutralizing the protease activity of its receptor, ACE2, S induces an increase in circulating Angiotensin II (AngII), resulting in monocytic release of reactive oxygen species (ROS) and programmed T cell death.

Objective: Here, we tested whether SARS-CoV-2 mRNA vaccines, known to cause the circulation of the vaccine antigen, S-protein receptor binding domain (RBD), might trigger the same cascade.

Sample Preparation and Processing for Quick, Universal, and Insightful Microbial Proteomics.

Next-generation shotgun proteomics is one of the most valuable tools for gaining insight into the function of organisms. By providing a list of peptides and abundance information, proteomics enables the identification of proteins, their quantities, posttranslational modifications, and localization. The most refined shotgun proteomics workflow involves protein extraction, trypsin digestion, ultrahigh-performance liquid chromatography coupled to high-resolution tandem mass spectrometry, and confident assignment of resulting spectra to peptide sequences.

Evaluation of the double-zone hemolysis (DZH) test for the detection of livestock-associated methicillin-resistant .

Unlabelled: Livestock-associated methicillin-resistant (LA-MRSA), such as clonal-complex (CC)398, are of clinical relevance due to their multi-drug resistance profiles, adding to the overall burden of MRSA in humans. The objective was to evaluate the double-zone hemolysis (DZH) test as a simple and reliable method for detecting LA-MRSA in the clinical microbiology laboratory. isolates assigned to CC398 ( = 183; 152 MRSA/31 methicillin-susceptible [MSSA]), CC1 ( = 44; MRSA), and other CCs ( = 144; 94 MRSA/50 MSSA) were investigated.

A Surveillance Study of Culturable and Antimicrobial-Resistant Bacteria in Two Urban WWTPs in Northern Spain.

Wastewater treatment plants (WWTPs) are hotspots for the spread of antimicrobial resistance into the environment. This study aimed to estimate the proportion of clinically relevant antimicrobial-resistant bacteria in two Spanish urban WWTPs, located in the region of La Rioja (Spain); Methods: Ninety-four samples (48 water/46 sludge) were collected and streaked on ten different selective media, in order to recover the culturable bacterial diversity with relevant resistance phenotypes: Extended-Spectrum β-Lactamase-producing / (ESBL-Ec/Kp), Carbapenem-resistant (CR-E), Methicillin-resistant (MRSA), and Vancomycin-resistant / (VR-/). Isolates were identified by MALDI-TOF and were tested for antimicrobial susceptibility using the disk diffusion method.