Cross-sectional survey of rural and nonrural veterinarians indicates similarities in intent to stay or leave practice and differences in community attachment and satisfaction.

Objective: The primary objective was to identify veterinary workforce characteristics and factors contributing to staying in or leaving a veterinary practice. A secondary objective was to compare workforce characteristics and factors related to retention between survey respondents working in rural versus nonrural areas.

Methods: Veterinarians were surveyed via a questionnaire distributed with the use of a state veterinary medical association listserv or veterinary program alumni listserv from February 2022 to January 2023 and social media postings on X (formerly known as Twitter) and Facebook in April 2022.

Issue when expressing a recombinant protein under the control of p in BY-2 cells.

Several recombinant proteins have been successfully produced in plants. This usually requires -mediated cell transformation to deliver the T-DNA into the nucleus of plant cells. However, some genetic instability may threaten the integrity of the expression cassette during its delivery via , especially when the protein of interest is toxic to the bacteria.

In vivo deglycosylation of recombinant glycoproteins in tobacco BY-2 cells.

Production of recombinant pharmaceutical glycoproteins has been carried out in multiple expression systems. However, N-glycosylation, which increases heterogeneity and raises safety concerns due to the presence of non-human residues, is usually not controlled. The presence and composition of N-glycans are also susceptible to affect protein stability, function and immunogenicity.

Engineering the -glycosylation pathway of for molecular pharming using CRISPR/Cas9.

Molecular pharming in plants offers exciting possibilities to address global access to modern biologics. However, differences in the -glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins. Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for and .

Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells.

This protocol describes a robust method to obtain transgenic Nicotiana tabacum BY-2 cells that produce glycoproteins of interest via Agrobacterium tumefaciens transformation. Compared to biolistics-based transformation, this procedure requires only standard laboratory equipment.