Publications by authors named "C N Stefanis"
Background: Hepatocellular carcinoma (HCC) exhibits an exceptional intratumoral heterogeneity that might influence diagnosis and outcome. Advances in digital microscopy and artificial intelligence (AI) may improve the HCC identification of liver cancer cells.
Aim: Two AI algorithms were designed to perform computer-assisted discrimination of tumour from non-tumour nuclei in HCC.
Article Synopsis
- Checkpoint immunotherapy struggles against non-immunogenic tumors like neuroblastoma due to low MHC class I expression and neoantigen burden, but inhibiting ERAP1 can enhance anti-tumor immune responses.
- A novel strategy combining genetic knockout of ERAP1 with the HDAC inhibitor entinostat was developed, leading to increased immunogenicity and making neuroblastoma more responsive to PD-1 therapy.
- Experimental methods included CRISPR/Cas9 gene editing, flow cytometry, and mass spectrometry to demonstrate that ERAP1 inhibition improves immune cell activity and increases MHC class I and PD-L1 expression in neuroblastoma cells, ultimately slowing tumor growth.
Article Synopsis
- Researchers are exploring extracellular vesicle (EV)-carried miRNAs as potential biomarkers for assessing tumor aggressiveness and tailoring therapies, due to their stability and tissue specificity.
- A study identified nine miRNAs significantly upregulated in RMS patients, with miR-335-5p showing promise as a marker for distinguishing ARMS from ERMS, correlating with disease severity and patient survival outcomes.
Total Quality Management (TQM) is a holistic approach widely adopted across industries to ensure quality control and management. This document examines TQM practices in the pharmaceutical, food, and nutritional supplement sectors, highlighting their vital role in public health, sustainability, and consumer acceptance. By analyzing the literature and case studies, the article demonstrates how TQM significantly ensures product safety and quality.
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- This study focuses on utilizing orange and lemon pomace, a by-product of fruit processing, to develop plant complexes that may treat inflammatory bowel disease (IBD).
- Food-grade extracts were created using ultrasound-assisted maceration, followed by extensive screening of their chemical properties and biological effects through various analytical techniques.
- Both orange and lemon extracts demonstrated significant protective effects against intestinal damage, oxidative stress, and inflammation in human cell models, suggesting their potential for further research in IBD treatment.