A dose escalation and safety study of AAVrh10-mediated Schwann cell-targeted gene therapy for CMT1X.

X-linked Charcot-Marie-Tooth disease (CMT1X) is an inherited demyelinating neuropathy caused by loss-of-function mutations in the GJB1 gene, encoding the gap junction protein connexin32 (Cx32). Cx32 plays a critical role in Schwann cell function and myelin formation in the peripheral nervous system. We have developed a gene replacement therapeutic approach using a humanized AAVrh10 vector construct expressing GJB1 under the control of the Schwann cell-specific human myelin protein zero (MPZ) promoter.

Post-processing enhances protein secondary structure prediction with second order deep learning and embeddings.

Protein Secondary Structure Prediction (PSSP) is regarded as a challenging task in bioinformatics, and numerous approaches to achieve a more accurate prediction have been proposed. Accurate PSSP can be instrumental in inferring protein tertiary structure and their functions. Machine Learning and in particular Deep Learning approaches show promising results for the PSSP problem.

Dietary Intake, Mediterranean and Nordic Diet Adherence in Alzheimer's Disease and Dementia: A Systematic Review.

Background/objectives: Dementia is not a single disease but an umbrella term that encompasses a range of symptoms, such as memory loss and cognitive impairments, which are severe enough to disrupt daily life. One of the most common forms of dementia is Alzheimer's Disease (AD), a complex neurodegenerative condition influenced by both genetic and environmental factors. Recent research has highlighted diet as a potential modifiable risk factor for AD.

Nutrient use and methane emissions in growing beef fed different protein sources and a pasture-based diet.

This study investigated the effects of different protein sources on feed intake, nutrient, and energy utilization, growth performance, and enteric methane (CH4) emissions in growing beef cattle, also evaluated against a pasture-based diet. Thirty-two Holstein × Angus growing beef were allocated to four dietary treatments: a total mixed ration (TMR) including solvent-extracted soybean meal as the main protein source (n = 8), TMR with local brewers' spent grains (n = 8), TMR with local field beans (n = 8), and a diet consisting solely of fresh-cut Italian ryegrass (GRA; n = 8). Every 4 wk, animals were moved to digestibility stalls within respiration chambers to measure nutrient intakes, energy and nitrogen (N) utilization, and enteric CH4 emissions.