Publications by authors named "C Mowery"
FOXP3 is a lineage-defining transcription factor that controls differentiation and maintenance of suppressive function of regulatory T cells (Tregs). Foxp3 is exclusively expressed in Tregs in mice. However, in humans, FOXP3 is not only constitutively expressed in Tregs; it is also transiently expressed in stimulated CD4+CD25- conventional T cells (Tconvs).
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- Researchers studied how cis-regulatory elements (CREs) work with trans regulators to control the expression of T cell genes CD28, CTLA4, and ICOS, which are important for immune responses.
- Using CRISPR interference (CRISPRi) screens, they identified specific CREs that vary depending on the type of T cell and stimulation, revealing the complexity of gene regulation.
- They found that the CCCTC-binding factor (CTCF) plays a key role in enhancing the interaction between CREs and CTLA4 while also preventing unintended activation of CD28, helping to clarify the regulatory landscape of these immune genes.
Chronic stimulation can cause T cell dysfunction and limit the efficacy of cellular immunotherapies. Improved methods are required to compare large numbers of synthetic knockin (KI) sequences to reprogram cell functions. Here, we developed modular pooled KI screening (ModPoKI), an adaptable platform for modular construction of DNA KI libraries using barcoded multicistronic adaptors.
View Article and Find Full Text PDFHLA-C*07:985:01:02Q differs from HLA-C*07:985:01 by one nucleotide substitution at the Intron 1 splicing acceptor site.
View Article and Find Full Text PDFObjective: Patients with incomplete lupus erythematosus (ILE) have lupus features but insufficient criteria for SLE classification. Some patients with ILE transition to SLE, but most avoid major organ involvement. This study tested whether the milder disease course in ILE is influenced by reduced SLE risk allele genetic load.
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