Gene Editing and Protein Tagging in the Oomycete Phytophthora infestans Using CRISPR-Cas12a.

Molecular genetic tools such as CRISPR-Cas gene editing systems are invaluable for understanding gene and protein function and revealing the details of a pathogen's life and disease cycles. Here we present protocols for genome editing in Phytophthora infestans, an oomycete with global importance as a pathogen of potato and tomato. Using a vector system that expresses variants of Cas12a from Lachnospiraceae bacterium and its guide RNA from a unified transcript, we first present a method for editing genes through the non-homologous end-joining (NHEJ) pathway.

Is Strength the Main Risk Factor of Overuse Shoulder Injuries? A Cohort Study of 296 Amateur Overhead Athletes.

Background: Shoulder pain is one of the most common musculoskeletal complaints in overhead athletes. This study investigated the prevalence of the main risk factors and sex differences related to the development of shoulder pain in a cohort of amateur overhead athletes.

Hypothesis: The external rotation/internal rotation (ER/IR) isometric strength ratio <0.

Modeling the potential public health and economic impact of different COVID-19 vaccination strategies with an adapted vaccine in the Kingdom of Saudi Arabia.

Background: The dynamic evolution of the virus causing COVID-19 necessitates the development of adapted vaccines to protect against emerging variants.

Research Design And Methods: A combined Markov-decision tree model estimated the outcomes of alternative vaccination strategies. The Saudi Arabian population was stratified into standard-risk and high-risk subpopulations, defined as either the population comprising individuals aged ≥ 65 years and individuals with at least one comorbidity.

Hazardous gas emissions from drop-in biofuels: mutagenicity, cytotoxicity, and unregulated pollutants.

This study investigates cancer-related mutations (TA98 and YG5185 strains/Ames test), cell death (human A549 cell line/MTT assay) and unregulated pollutants (16 PAH, 13 carbonyls) from the gas exhaust emissions from a last-mile delivery vehicle following the WLTC driving cycle, operating with hydrotreated vegetable oil and biodiesel. Both biofuels were used pure and blended 20 % by volume with diesel fuel. Gas phase samples were collected using XAD-2 Amberlite® resin.