Effect of DNA extraction method in gut fungal community assessment.

Understanding the gut mycobiota composition and its impact on health requires reliable methods for fungal community assessment. This study explores the influence of DNA extraction methods in GM analysis. Three protocols were evaluated: Qiagen DNeasy blood and tissue kit with mechanical glass bead lysis (DNgb), Thermofisher MagMax Microbiome ultra-nucleic isolation kit automated method (MM), and MM combined with glass beads lysis (MMgb).

Cognitive function at first episode in patients subsequently developing treatment-resistant schizophrenia.

Background: Research on cognitive functions in treatment-resistant schizophrenia (TRS) has focused on chronic patients, complicating the distinction between disease-related deficits from those influenced by chronicity or antipsychotic exposure. Identifying early cognitive differences could offer insights into the nature of TRS cognitive performance and serve as potential markers of treatment resistance.

Methods: Cohort study of 81 first-episode schizophrenia patients from Chile.

Impact of light and nutrient availability on the phagotrophic activity of harmful bloom-forming dinoflagellates.

Phagotrophy is a key nutritional mode for many bloom-forming dinoflagellates that can supplement their carbon and nutrient requirements. However, the environmental drivers and ecological relevance of phagotrophy in algal blooms are still poorly understood. This study evaluates the effect of light and nutrient availability on the phagotrophic activity of three common bloom-forming dinoflagellates (, and ) using three fluorescently labeled preys: bacteria, and the haptophyte .

Cutaneous Innate Lymphoid Populations Drive IL-17A-Mediated Immunity in Nannizzia gypsea Dermatophytosis.

Fungal skin infections significantly contribute to the global human disease burden, yet our understanding of cutaneous immunity against dermatophytes remains limited. Previously, we developed a model of epicutaneous infection with Microsporum canis in C57BL/6 mice, which highlighted the critical role of IL-17RA signaling in antidermatophyte defenses. In this study, we expanded our investigation to the human pathogen Nannizzia gypsea and demonstrated that skin γδTCR and CD8/CD4 double-negative βTCR T cells are the principal producers of IL-17A during dermatophytosis.

[The Public Health Services at the Metropolitan Region (MR) of Chile have nine acute psychiatric beds per 100,000 over 18 years old inhabitants, insufficient for current need].

Background: The Public Health Services at the Metropolitan Region (MR) of Chile have nine acute psychiatric beds per 100,000 inhabitants, under international recommendations.

Aim: The present study will evaluate the resolution capacity of the main MR Psychiatric Emergency Room (PER), which may help assess the impact of the availability of acute beds in the MR.

Material And Methods: A retrospective observational study of electronic patient records for all adult patients attending PER of the Psychiatric Institute "Dr.