Publications by authors named "C M Verma"

Real time-polymerase chain reaction (RT-PCR) is used routinely in clinical practice as a cost-effective method for molecular diagnostics. Research in pediatric B-cell Acute Lymphoblastic Leukemia (ped B-ALL) suggests that apart from cytogenetics and clinical features, there is a need to include Copy number variation (CNV) in select genes at diagnosis, for upfront stratification of patients. Using ped B-ALL as a model, we have developed a RT-PCR-based iterative probability scoring method for reporting CNVs, and relative gene-expression changes.

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Introduction: Efficient extraction of DNA from biological fluids is crucial for applications in molecular biology, forensic science, and clinical diagnostics. However, traditional DNA extraction methods often require costly reagents and lengthy procedures. This study aims to optimize the binding buffer composition for DNA extraction using polyethyleneimine-coated iron oxide nanoparticles (PEI-IONPs), which offer the dual benefits of magnetic separation and high DNA-binding efficiency.

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This work involves the synthesis of two chitosan derivatives by reacting chitosan, extracted from shrimp shells in eastern Morocco, with 2-nitrobenzaldehyde via a Schiff base reaction. An amino derivative of chitosan was then produced by reducing the imine group created by sodium borohydride. We investigated the molecular weight (), crystallinity index (), and degree of deacetylation () of the isolated chitosan, among other characteristic features.

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Article Synopsis
  • * Involving 200 newly diagnosed HIV-1 patients, the research revealed significant correlations, such as lower hemoglobin levels linked to higher CD4+ counts and lower PVL, while abnormalities in various blood parameters were more pronounced in those co-infected with HIV-TB.
  • * The findings suggest that specific hematological and biochemical indicators, including hemoglobin and inflammatory markers, could serve as early warning signs for TB in HIV-infected individuals, enhancing patient management
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