Publications by authors named "C M Kieffer"
The circulation of seasonal influenza A viruses (IAVs) in humans relies on effective evasion and subversion of the host immune response. While the evolution of seasonal H1N1 and H3N2 viruses to avoid humoral immunity is well characterized, relatively little is known about the evolution of innate immune antagonism phenotypes in these viruses. Numerous studies have established that only a small subset of infected cells is responsible for initiating the type I and type III interferon (IFN) response during IAV infection, emphasizing the importance of single cell studies to accurately characterize the IFN response during infection.
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- XIAP is a protein that helps regulate cell survival and inflammation, and targeting it could benefit diseases like Crohn's disease and sarcoidosis.
- Researchers developed selective inhibitors for a specific part of XIAP (XIAP-BIR2) to disrupt its interaction with RIPK2, which is relevant to inflammatory diseases.
- They created a library of small synthetic molecules, identifying compound 20c, which effectively blocks the NOD1/2 signaling pathway in cells and holds potential as an anti-inflammatory treatment.
The circulation of seasonal influenza A viruses (IAVs) in humans relies on effective evasion and subversion of the host immune response. While the evolution of seasonal H1N1 and H3N2 viruses to avoid humoral immunity is well characterized, relatively little is known about the evolution of innate immune antagonism phenotypes in these viruses. Numerous studies have established that only a small subset of infected cells is responsible for initiating the type I and type III interferon (IFN) response during IAV infection, emphasizing the importance of single cell studies to accurately characterize the IFN response during infection.
View Article and Find Full Text PDFHIV latency regulation in monocytes and macrophages can vary according to signals directing differentiation, polarization, and function. To investigate these processes, we generated an HIV latency model in THP-1 monocytes and showed differential levels of HIV reactivation among clonal populations. Monocyte-to-macrophage differentiation of HIV-infected primary human CD14+ and THP-1 cells induced HIV reactivation and showed that virus production increased concomitant with macrophage differentiation.
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- - Exponential DNA amplification techniques are crucial for highly sensitive molecular diagnostics, but they require real-time monitoring, whereas linear amplification methods, though less sensitive, allow for simple end-point measurements ideal for cost-effective testing.
- - The new CELIA (Coupling Exponential amplification reaction to Linear Amplification) design merges exponential and linear amplification in a single process, enabling quantification through a single end-point measurement while maintaining accuracy across a vast dynamic range.
- - This innovative method, particularly effective in detecting microRNAs, offers high sensitivity and a wide range of detection without the need for complex thermocyclers, making it a practical solution for various diagnostic applications and improving efficiency in quantitative nucleic acid analysis.