Development of an in vitro fibrin clot model to evaluate fibrinolytic agents for wound care application.

Objective: To describe an in vitro fibrin clot model that could reliably assess the fibrinolytic activity of enzymatic debriding agents for wound care application.

Method: A model of a fibrin clot was reconstructed in vitro by mixture of human fibrinogen and (alpha)-thrombin supplemented with factor XIII. These clots were then treated with enzymatic ointments.

Stimulation of the proliferation of human dermal fibroblasts in vitro by a lipidocolloid dressing.

Objective: The effect of Urgotul on normal human dermal fibroblast proliferation was studied in vitro and compared with that of two other dressings: Mepitel and Tulle Gras.

Method: Proliferation was measured by the extent of thymidine incorporation into the replicating DNA of proliferative fibroblasts in contact with the complete dressing.Additional cell viability and metabolism were evaluated using MTT assay.