Publications by authors named "C Lambrecht"
Article Synopsis
- High-grade cervical lesions and cancers (HSIL+) can sometimes test negative for HPV, leading to variability in HPV detection rates among labs.
- A study re-analyzed 116 HPV-negative cervical specimens, discovering that over 61% actually contained HPV upon further testing.
- The findings suggest that improving quality assurance measures and re-analysis protocols for HPV-negative HSIL+ cases can enhance the reliability of HPV testing services.
Porcine viruses have been emerging in recent decades, threatening animal and human health, as well as economic stability for pig farmers worldwide. Next-generation sequencing (NGS) can detect and characterize known and unknown viruses but has limited sensitivity when an unbiased approach, such as shotgun metagenomics sequencing, is used. To increase the sensitivity of NGS for the detection of viruses, we applied and evaluated a broad viral targeted sequence capture (TSC) panel and compared it to an unbiased shotgun metagenomic approach.
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- Porcine respirovirus 1 (PPIV-1) was first identified in Hong Kong in 2013 and has since spread to countries like the USA, Chile, and Hungary, with limited information on its impact.
- A study in the Dutch-German border region used shotgun metagenomics sequencing to analyze 53 pig samples, confirming PPIV-1 in 1 of 5 farms initially found positive and discovering 6 more infected farms.
- Phylogenetic analysis indicated a close relationship to the original Hong Kong strain, suggesting PPIV-1 is widely circulating in Central Europe and emphasizing the need for more research on its effects and transmission.
Shotgun metagenomic sequencing (SMg) enables the simultaneous detection and characterization of viruses in human, animal and environmental samples. However, lack of sensitivity still poses a challenge and may lead to poor detection and data acquisition for detailed analysis. To improve sensitivity, we assessed a broad scope targeted sequence capture (TSC) panel (ViroCap) in both human and animal samples.
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