Publications by authors named "C L Sterling"
The murine hepatitis virus (MHV) is an important model system for studying coronavirus (CoV) molecular and cell biology. Despite this, few reagents for MHV are available through repositories such as ATCC or Addgene, potentially limiting the widespread adoption of MHV as a tractable model system. To overcome some challenges inherent in the existing MHV reverse genetics systems, we developed a plasmid-launched transformation-associated recombination (TAR) cloning-based system to assemble the MHV (strain A59; MHV-A59) genome.
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- Improved Overall Survival (OS) and Progression-Free Survival (PFS): Patients with relapsed classic Hodgkin lymphoma (cHL) who received immune checkpoint inhibitors (ICI) before undergoing allogeneic bone marrow transplantation (alloBMT) had significantly better 5-year OS (91%) and PFS (84%) compared to those treated with chemotherapy without ICI (66% OS and 53% PFS).
- GVHD Incidence and Management: The study found that the incidence of severe graft-versus-host disease (GVHD) was higher in patients who received ICI prior to alloBMT, with a 12-month incidence of grade III-IV GVHD at 20% for the ICI group versus 7%
Unlabelled: Patients who have radiographically detectable lesions in their brain or other symptoms compatible with brain tumors pose challenges for diagnosis. The only definitive way to diagnose such patients is through brain biopsy, an obviously invasive and dangerous procedure. Here we present a new workflow termed "CSF-BAM" that simultaneously identifies cell or T cell receptor rearrangements, neuploidy, and using PCR-mediated amplification of both strands of the DNA from CSF samples.
View Article and Find Full Text PDFPhosphoinositide (PIP) signaling plays pivotal roles in myriad biological processes and is altered in many diseases including cancer. Canonical PIP signaling involves membrane-associated PIP lipid second messengers that modulate protein recruitment and activity at membrane focal points. In the nucleus, PIP signaling operates separately from membranous compartments defining the paradigm of non-canonical PIP signaling.
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- Jamestown Canyon virus (JCV) is a type of bunyavirus that causes neuroinvasive diseases in the U.S., but its mechanism of infection is not well understood.
- Recent research identifies low-density lipoprotein receptor related protein 1 (Lrp1) as a key factor that enables JCV and other bunyaviruses to infect cells, particularly in the nervous system.
- Experiments show that neurons lacking Lrp1 have reduced JCV infection rates, suggesting that targeting Lrp1 could lead to new antiviral treatments for diseases caused by bunyaviruses.