Publications by authors named "C L Mummery"

Article Synopsis
  • Brugada Syndrome (BrS) is a serious heart condition that can cause sudden cardiac death and is linked to mutations in the SCN5A gene, which affects sodium channels in the heart.
  • Researchers created human induced pluripotent stem cell (hiPSC) lines with a specific BrS mutation (c.845G>A or p.R282H) to better understand its effects.
  • The study compares two hiPSC lines: one with the mutation on the same gene copy as another polymorphism (c.1673A>G or p.H558R) and one with it on the opposite gene copy, providing a model to investigate their physiological impacts.
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Targeting DNA payloads into human (h)iPSCs involves multiple time-consuming, inefficient steps that must be repeated for each construct. Here, we present STRAIGHT-IN Dual, which enables simultaneous, allele-specific, single-copy integration of two DNA payloads with 100% efficiency within one week. Notably, STRAIGHT-IN Dual leverages the STRAIGHT-IN platform to allow near-scarless cargo integration, facilitating the recycling of components for subsequent cellular modifications.

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Skeletal muscle spatial analyses have revealed unexpected regionalized gene expression patterns challenging the understanding of muscle as a homogeneous tissue. Here, we present a protocol for the spatial analysis of transcript and protein levels in murine skeletal muscle. We describe steps for tibialis anterior dissection, formaldehyde fixation, tissue chopper cutting, and hybridization chain reaction (HCR) detection and amplification.

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Cardiomyocytes in the adult human heart are quiescent and those lost following heart injury are not replaced by proliferating survivors. Considerable effort has been made to understand the mechanisms underlying cardiomyocyte cell cycle exit and re-entry, with view to discovering therapeutics that could stimulate cardiomyocyte proliferation and heart regeneration. The advent of large compound libraries and robotic liquid handling platforms has enabled the screening of thousands of conditions in a single experiment but success of these screens depends on the appropriateness and quality of the model used.

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