Transport of long chain fatty acids in Escherichia coli. Identification of a membrane protein associated with the fadL gene.

The fadL gene is required for the transport of long chain fatty acids in Escherichia coli (Maloy, S. R., Ginsburgh, C.

Isolation and genetic characterization of Escherichia coli mutants defective in propionate metabolism.

Escherichia coli mutants defective in propionate metabolism (Prp-) were isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Prp- mutants demonstrate a phenotypic inability to grow on odd-chain-length fatty acids. The new genetic locus for the Prp- phenotype maps at approximately 98 min on the E.

Transport of long and medium chain fatty acids by Escherichia coli K12.

Kinetic, metabolic, and physical parameters of long and medium chain fatty acid transport by Escherichia coli K12 were determined. Uptake of long chain fatty acids (C11-C18:1) mediated by the fadL gene involves concentrative transport. Evidence for this is as follows: (i) characteristic Ki and Vmax values were obtained for long chain fatty acids, (ii) long chain fatty acid transport was inhibited by energy inhibitors, (iii) long chain fatty acids were concentrated 10-fold inside the cell against a concentration gradient, (iv) efflux of transported long chain fatty acids did not occur, and (v) an energy of activation of 11.

Independent localization and regulation of carbamyl phosphate synthetase A polypeptides of Neurospora crassa.

Carbamyl phosphate synthetase A is a two-polypeptide, mitochondrial enzyme of arginine synthesis in Neurospora. The large subunit is encoded in the arg-3 locus and can catalyze formation of carbamyl-P with ammonia as the N donor. The small subunit is encoded in the unlinked arg-2 locus and imparts to the holoenzyme the ability to use glutamine, the biological substrate, as the N donor.

Sources of subcortical projections to the superior colliculus in the cat.

A comprehensive search for subcortical projections to the cat superior colliculus was conducted using the retrograde horseradish peroxidase (HRP) method. Over 40 different subcortical structures project to the superior colliculus. The more notable among these are grouped under the following categories.