Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples.
View Article and Find Full Text PDFEstimates of abundance are essential to manage and conserve marine species. Numerous methods are available to determine population size, but the suitability of methods for schooling fishes and the associated precision can vary depending on the species and system. Here, we developed and compared three mark-recapture/resight methods to assess the most robust method to estimate the abundance of silver trevally (Pseudocaranx georgianus).
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