The Distribution of Gibberellins in Vegetative Tissues of Pisum sativum L. : I. Biological and Biochemical Consequences of the le Mutation.

The concentrations of endogenous gibberellin (GA) 1, 5, 8, 19, 20, and 29 in the component tissues of maturing tall (Le) and dwarf (le) pea (Pisum sativum) plants have been determined. The following conclusions were drawn from the data obtained: (a) GA(20) and its metabolites accumulate only in the growing regions of Le and le plants; (b) the le mutation is biochemically expressed in all immature tissues of the dwarf plants; (c) the quantitative composition of the GA metabolites in the various immature tissues is variable; (d) the total GA concentration in apical buds, unexpanded leaves, and tendrils is considerably higher than in GA(1)-responsive stem tissue; and (e) there is very little GA accumulation of the inactive 2beta-hydroxylated GAs (GA(8) and GA(29)) in either the mature vegetative tissues or the roots of pea plants.

Immunochromatographic purification of gibberellins from vegetative tissues of Cucumis sativus L: Separation and identification of 13-hydroxy and 13-deoxy gibberellins.

Immunological methods are described for the separation and purification of 13-hydroxy and 13-deoxy-gibberellins of Cucumis sativus. Qualitative and quantitative data show that 13-deoxygibberellins predominate over 13-hydroxygibberellins in stems and leaves of this species.