Cryopreservation of murine ovarian tissue.

To determine the feasibility of ovarian transplantation using cryopreserved tissue, ovaries were removed from anesthetized 12- to 20-week-old female mice. The controls consisted of a sham-operated group and an oophorectomized group, and the experimental groups were autologous transplants with and without cryopreservation. Ovaries for cryopreservation were suspended in 1.