Raman Handheld Versus Microscopic Spectroscopy for Estimating the Post-Mortem Interval of Human Bones: A Comparative Pilot Study.

The post-mortem interval estimation for human skeletal remains is critical in forensic medicine. This study used Raman spectroscopy, specifically comparing a handheld device to a Raman microscope for PMI estimations. Analyzing 99 autopsy bone samples and 5 archeological samples, the research categorized them into five PMI classes using conventional methods.

Exploring near-infrared spectroscopy and hyperspectral imaging as novel characterization methods for anaerobic gut fungi.

Neocallimastigomycota are a phylum of anaerobic gut fungi (AGF) that inhabit the gastrointestinal tract of herbivores and play a pivotal role in plant matter degradation. Their identification and characterization with marker gene regions has long been hampered due to the high inter- and intraspecies length variability in the commonly used fungal marker gene region internal transcribed spacer (ITS). While recent research has improved methodology (i.

Phytochemical profiling of oak bark extract: A combined approach using near-infrared spectroscopy and liquid chromatography-mass spectrometry.

The aim of the presented study was to qualitatively and quantitatively determine the chemical composition of oak bark extracts in order to gain insights into the effectiveness as alternative medication for various diseases. The primary emphasis was on developing a near-infrared spectroscopy (NIRS) method for precise quantification of two key polyphenolic compounds, specifically gallic acid and catechin, in form of a fast and non-destructive quality control. A comprehensive dataset consisting of 48 samples from various production batches was analyzed throughout this research.

Species-Specific quantification of bioactive boswellic acids in Boswellia resin using NIR spectroscopy, HPLC and Multivariate data analysis.

The bioactive compounds Acetyl-11-keto-β-boswellic acid (AKBA) and 11-keto-β-boswellic acid (KBA), found in the resin of the Boswellia tree, exhibit anti-inflammatory properties, rendering Boswellia resin an intriguing natural medicinal products. However, the content of boswellic acids varies across different Boswellia species and proper knowledge of its species-dependent nature, as well as alternatives to the resource- and time-intensive HPLC analysis, are lacking. Here we present a comprehensive investigation into the boswellic acid content of seven Boswellia species from ten countries and introduce a novel and non-destructive Near-Infrared spectroscopy method for predicting boswellic acid concentrations in solid resin samples.

Suitability of Fourier transform infrared microscopy for the diagnosis of cystic echinococcosis in human tissue sections.

Cystic echinococcosis (CE) is a global health concern caused by cestodes, posing diagnostic challenges due to nonspecific symptoms and inconclusive radiographic results. Diagnosis relies on histopathological evaluation of affected tissue, demanding comprehensive tools. In this retrospective case study, Fourier transform infrared microscopy was explored for detecting and identifying CE through biochemical changes in human tissue sections.