Bone marrow stromal cell antigen 2 is broadly expressed in the different pluripotent states of human pluripotent stem cells and regulates the expression of pluripotency genes and three germ layer markers.

Human pluripotent stem cells (hPSCs) have at least three distinct states: naïve pluripotency that represents the cellular states of the pre-implantation epiblast cells, primed pluripotency that represents the cellular states of the post-implantation epiblast cells, and formative pluripotency that represents a developmental continuum between naïve and primed pluripotency. Various cell surface markers have been used to define and analyze primed and naïve hPSCs within heterogeneous populations. However, not much is known about common cell surface markers for the different pluripotent states of hPSCs.

Gas-Liquid-Solid Three-Phase Boundary in Scanning Electrochemical Cell Microscopy.

The gas-liquid-solid interface plays a crucial role in various electrochemical energy conversion devices, including fuel cells and electrolyzers. Understanding the effect of gas transfer on the electrochemistry at this three-phase interface is a grand challenge. Scanning electrochemical cell microscopy (SECCM) is an emerging technique for mapping the heterogeneity in electrochemical activity; it also inherently features a three-phase boundary at the nanodroplet cell.

Assessment of surface contamination of low-dose radioactive iodine (131I) treatment container.

With the advancement of radiotherapy technology in the medical field, the amount of radioactive waste has rapidly increased, and the International Atomic Energy Agency (IAEA) has proposed waste deregulation standards based on individual dose, collective dose, and nuclide concentration. The purpose of this study is to define the standard period (1 day) required to measure collected radioactive waste using direct and indirect methods with a radioactivity meter, ensure that the radiation dose remains below the allowable level, and transport the waste safely. In this study, 131I low-dose (30 mCi) radioactive waste discarded after radioiodine treatment at a medical institution was collected, and a measuring container was prepared to measure radioactivity concentration according to IAEA standards.

Lipid Nanoparticle-Mediated CRISPR-Cas13a Delivery for the Control of Bacterial Infection.

Lipid nanoparticles (LNPs) can assist in the delivery of nucleic acid inside animal cells, as demonstrated by their use in COVID-19 vaccine development. However, LNPs applicable to bacteria have not been reported. Here, the screening of 511 LNPs containing random combinations of different lipid components identified two LNPs, LNP 496 and LNP 470, that efficiently delivered plasmids into Escherichia coli BW25113.