The induction of aberrant crypt foci (ACF) in the colons of rats by trihalomethanes administered in the drinking water.

Bromodichloromethane (BDCM) and bromoform (TBM) have been demonstrated to be colon carcinogens in male and female F344/N rats following administration by corn oil gavage. Our chronic bioassay of BDCM administered in the drinking water failed to demonstrate colon cancer in male F344/N rats. In the present study we addressed the capability of trihalomethanes (THMs) administered in drinking water to induce aberrant crypt foci (ACF), early putative preneoplastic lesions, in the colons of male F344/N rats and B6C3F(1) mice.

Virus isolation accuracy: an evaluation of eight laboratories involved in poultry diagnostics.

A study was undertaken to evaluate the accuracy of diagnostic laboratories at isolation of common avian viral pathogens. Fourteen "unknown" samples were submitted to eight laboratories in seven states. All positive and negative samples were guaranteed pure by SPAFAS.

A survey for parvovirus-like virus (so-called chick anemia agent) antibodies in broiler breeders.

A prospective study to survey for the presence of parvovirus-like virus (PVLV; so-called chick anemia agent) antibody in broiler breeder pullets in Georgia, North Carolina, and Florida was conducted by collecting serum samples from 52 breeder flocks that ranged in age from 1 day to 55 weeks old. Results indicated that PVLV infection was widespread. Ninety-eight percent (51/52) of chicken flocks and 62% (530/861) of chickens had PVLV antibody.

Preparation of an agar-gel-precipitating antigen for avian encephalomyelitis and its use in evaluating the antibody status of poultry.

An agar-gel-precipitin (AGP) antigen for avian encephalomyelitis virus (AEV) was prepared from infected chicken embryo brains. The antigen could precipitate specific antibodies to AEV. No nonspecific reactions were observed.

Serologic detection of adenovirus infections in specific-pathogen-free chickens.

A specific-pathogen-free flock of White Leghorns, which were housed conventionally and were previously serologically negative for all common poultry pathogens including avian adenoviruses, incurred an outbreak of adenovirus that was detected at about 39 weeks of age. The infection was detected serologically through the agar-gel precipitin test (AGPT) and also by a microneutralization test (MNT) adapted for 11 serotypes of avian adenovirus. The MNT detected specific antibodies to serotype-3 avian adenovirus (IBHV-Tipton) but no other serotype.