Prediction of the octanol-water partition constant of neutral compounds by reversed-phase liquid chromatography using alternative organic solvent modifiers.

Reversed-phase liquid chromatography (RPLC) offers significant advantages over traditional methods for estimating octanol-water partition constants, which are a critical parameter in drug discovery. In contrast to classical methods for determining the octanol-water partition constant, such as shake-flask techniques, RPLC is less time-consuming and easier to automate. In this study, we explored three alternative organic solvent modifiers: acetone, 2-propanol, and tetrahydrofuran for the indirect determination of the octanol-water partition constant for neutral compounds by RPLC using either isocratic retention factors or retention factors extrapolated to 100 % water for several stationary phases.

Four-Dimensional Flow MRI for Cardiovascular Evaluation (4DCarE): A Prospective Non-Inferiority Study of a Rapid Cardiac MRI Exam: Study Protocol and Pilot Analysis.

Background: Accurate measurements of flow and ventricular volume and function are critical for clinical decision-making in cardiovascular medicine. Cardiac magnetic resonance (CMR) is the current gold standard for ventricular functional evaluation but is relatively expensive and time-consuming, thus limiting the scale of clinical applications. New volumetric acquisition techniques, such as four-dimensional flow (4D-flow) and three-dimensional volumetric cine (3D-cine) MRI, could potentially reduce acquisition time without loss in accuracy; however, this has not been formally tested on a large scale.

The repeatability of behavioural laterality during nest building in zebra finches.

Cerebral laterality is a widespread phenomenon across animals and refers to the specialization of the left and right hemispheres of the brain for perceptual, cognitive and behavioural tasks. Behavioural laterality occurs in several contexts, including foraging, mate selection, predator detection and tool manufacture. Behavioural laterality during nest building, however, has rarely been addressed.

Predicting biophysical properties of small molecules from chromatographic measurements and the solvation parameter model.

Biopartitioning processes are challenging to study and often require the sacrifice of multiple animals. Therefore, it is more practical and cost-effective to correlate these processes with easily determined properties, such as chromatographic retention data, or to make predictions based on structural descriptors such as quantitative structure-property relationships or linear free energy relationships. Abraham's solvation parameter model uses six solute properties to characterize the interactions responsible for the transfer of neutral compounds between immiscible phases in chromatographic or biological systems.

Cell Population-resolved Multi-Omics Atlas of the Developing Lung.

The lung is a vital organ that undergoes extensive morphological and functional changes during postnatal development. To disambiguate how different cell populations contribute to organ development, we performed proteomic and transcriptomic analyses of four sorted cell populations from the lung of human subjects aged 0 to 8 years-old with a focus on early life. The cell populations analyzed included epithelial, endothelial, mesenchymal, and immune cells.