Heterotrimeric G protein signaling functions with dynein to promote spindle positioning in C. elegans.

Proper orientation and positioning of the mitotic spindle is essential for the correct segregation of fate determinants during asymmetric cell division. Although heterotrimeric G proteins and their regulators are essential for spindle positioning in many cell types, their mechanism of action remains unclear. In this study, we show that dyrb-1, which encodes a dynein light chain, provides a functional link between heterotrimeric G protein signaling and dynein activity during spindle positioning in Caenorhabditis elegans.

Control of embryonic spindle positioning and Galpha activity by C. elegans RIC-8.

Asymmetric spindle positioning is of fundamental importance for generating cell diversity during development. In the C. elegans 1 cell embryo, spindle positioning has been shown to depend on heterotrimeric G protein signaling.

Acute effects of drinking beer or wine on the steroid hormones of healthy men.

Plasma Testosterone, Dihydrotestosterone and Estradiol were determined in 72 healthy volunteers, divided into 36 beer and 36 wine drinkers, who consumed between 0.9-2.1 g ethanol/kg b.

Relations between sex hormone levels and sexual behavior in men.

Using a sample of 33 healthy young men, 6 blood samples were obtained in the course of 2 weeks, and testosterone, dihydrotestosterone, and estradiol levels determined. The amount of free testosterone in the saliva was also ascertained for 23 of the subjects. All participants kept a daily record of their sexual activity during the investigational period.

Comparisons of the intra- and inter-individual variability in sex hormone levels of men.

Six blood samples were taken from each of 33 healthy young men during the course of 2 weeks. From these, the testosterone, dihydrotestosterone, and oestradiol levels were determined. In addition, free testosterone was measured using saliva samples obtained from 23 of the subjects.