Publications by authors named "C J Allan"
Objective: We have shown that men aged 50 years+ at high risk of type 2 diabetes treated with testosterone together with a lifestyle program reduced the risk of type 2 diabetes at 2 years by 40% compared to a lifestyle program alone. To develop a personalized approach to treatment, we aimed to explore a prognostic model for incident type 2 diabetes at 2 years and investigate biomarkers predictive of the testosterone effect.
Design: Model development in 783 men with impaired glucose tolerance but not type 2 diabetes from Testosterone for Prevention of Type 2 Diabetes; a multicenter, 2-year trial of Testosterone vs placebo.
Introduction: Long COVID is a debilitating condition that lasts for more than three months post-infection by SARS-CoV-2. On average, one in ten individuals infected with SARS CoV- 2 develops Long COVID worldwide. A knowledge gap exists in our understanding of the mechanisms, genetic risk factors, and biomarkers that could be associated with Long COVID.
View Article and Find Full Text PDFMolecular alterations in cancerous tissues exhibit intercellular genetic and epigenetic heterogeneity, complicating the performance of diagnostic assays, particularly for early cancer detection. Conventional liquid biopsy methods have limited sensitivity and/or ability to assess epigenetic heterogeneity of rare epiallelic variants cost-effectively. We report an approach, named REM-DREAMing (Ratiometric-Encoded Multiplex Discrimination of Rare EpiAlleles by Melt), which leverages a digital microfluidic platform that incorporates a ratiometric fluorescence multiplex detection scheme and precise digital high-resolution melt analysis to enable low-cost, parallelized analysis of heterogeneous methylation patterns on a molecule-by-molecule basis for the detection of cancer in liquid biopsies.
View Article and Find Full Text PDFArticle Synopsis
- Plants adapt to environmental stress by altering growth and development, with calcium (Ca) playing a crucial role as a secondary messenger in response to these stressors.
- The bi-directional dual-flow RootChip (bi-dfRC) platform was used to study root growth and defense signaling by simulating salinity stress with sodium chloride (NaCl) treatment, revealing a concentration-dependent calcium burst that spreads from the stele to the root tip.
- Research also demonstrated that salinity impacts root growth differently depending on root architecture, with varying responses observed and novel insights gained into root force sensing through engineered microfluidic channels.
Mismatch between lab-generated and field-evolved resistance to transgenic Bt crops in .
Proc Natl Acad Sci U S A
November 2024
Article Synopsis
- Transgenic crops that produce Cry proteins, derived from the bacterium Bt, are widely used to combat key crop pests like the noctuid moth, but resistance to these proteins, particularly Cry1Ac, has been developing in pest populations.
- A study investigated the genetic basis of this field-evolved resistance in moth populations from various locations in the southern U.S. and found extensive gene mixing among them.
- Unlike previous lab findings, the resistance was linked to an increase in a cluster of nine trypsin genes rather than specific mutations in known resistance genes, indicating that there may be multiple genetic factors at play in the development of resistance.