Oligonucleotide microarray for the identification of carbapenemase genes of molecular classes a, B, and d.

This work is a report on the development of a method of hybridization analysis on DNA microarrays for the simultaneous identification and typing of carbapenemase-encoding genes. These enzymes are produced by the microorganisms that are responsible for causing infectious diseases. The method involves several steps, including DNA extraction from clinical samples and amplification of carbapenemase genes by multiplex PCR with simultaneous labelling by biotin.

[The effect of DNAse I on bacterial growth depending on the cultivation conditions and on the physiological characteristics of the inoculate].

The stimulating effect of DNAase 1 on Escherichia coli reproduction has been studied depending on the content of slowly growing cells in the inoculation culture in the phase of delayed growth. Three cell fractions of E. coli have been obtained using the stepwise separation of the population in terms of buoyant density in the phase of delayed growth.

Prothymosin alpha is an evolutionary conserved protein covalently linked to a small RNA.

A 13 kDa protein, covalently linked to a small RNA from the cytoplasm of mouse cells, was studied. Sequence analysis of its tryptic peptides revealed that the RNA-linked protein is identical to prothymosin alpha. Very similar RNA-protein complexes were identified in human, bovine and yeast cells.