Nanopore Sequencing for T-Cell Receptor Rearrangement Analysis in Cutaneous T-Cell Lymphoma.

Analysis of T-cell receptor (TCR) clonality is a major diagnostic tool for lymphomas, particularly for cutaneous T-cell lymphomas (CTCL) like Mycosis fungoides and Sézary syndrome. However, a fast and cost-effective workflow is needed to enable widespread use of this method. : We established a procedure for TCR rearrangement analysis via Oxford Nanopore Technology (ONT) sequencing.

A specific gFET-based aptasensor for quantitative monitoring of microbiome quality.

The use of health-relevant bacteria originating from human microbiomes for the control or therapy of diseases, including neurodegenerative disorders or diabetes, is currently gaining increasing importance in medicine. Directed and successful engineering of microbiomes probiotic supplementation requires subtle, precise as well as, more importantly, easy, fast and convenient monitoring of its success, , in patients' gut. Based on a previously described polyclonal SELEX aptamer library evolved against the human gut bacterium , we finally isolated three individual aptamers that proved their performance concerning affinity, specificity and robustness in reliably labeling the target bacterium and in combination with "contaminating" control bacteria.

Energy-Resolving Time-of-Flight Mass Spectrometry for Bulk Plasma Analysis.

This work presents a newly designed energy-resolving time-of-flight mass spectrometer (E-TOFMS) for analysing the energy and mass of ions in bulk plasma. The system comprises an electrostatic sector analyser (ESA) for energy-to-charge (E/Q) ratio resolution and an orthogonal reflectron TOFMS for mass-to-charge (m/Q) ratio analysis. The design choices are explained, providing insight into electron and ion path simulations.

Long-read sequencing and structural variant characterization in 1,019 samples from the 1000 Genomes Project.

Structural variants (SVs) contribute significantly to human genetic diversity and disease . Previously, SVs have remained incompletely resolved by population genomics, with short-read sequencing facing limitations in capturing the whole spectrum of SVs at nucleotide resolution . Here we leveraged nanopore sequencing to construct an intermediate coverage resource of 1,019 long-read genomes sampled within 26 human populations from the 1000 Genomes Project.

Nanostructure and Optical Property Tailoring of Zinc Tin Nitride Thin Films through Phenomenological Decoupling: A Pathway to Enhanced Control.

This work addresses the need for precise control of thin film sputtering processes to enable thin film material tailoring on the example of zinc tin nitride (ZTN) thin films deposited microwave plasma-assisted high power reactive magnetron sputtering (MAR-HiPIMS). The applied diagnostic techniques (Langmuir probe and energy-resolved time-of-flight mass spectrometry) supported monitoring changes in the deposition environment with respect to microwave (MW) power. During MAR-HiPIMS, the presence of nitride ions in the gas phase (ZnN, ZnN, SnN, SnN) was detected.