Publications by authors named "C Hagen"
Archived FFPE cardiac tissue specimens are valuable for molecular studies aimed at identifying biomarkers linked to mortality in cardiovascular disease. Establishing a reliable and reproducible RNA extraction method is critical for generating high-quality transcriptome sequences for molecular assays. Here, the efficiency of four RNA extraction methods: Qiagen AllPrep DNA/RNA method (Method QP); Qiagen AllPrep DNA/RNA method with protocol modification on the ethanol wash step after deparaffinization (Method QE); CELLDATA RNA extraction (Method BP) and CELLDATA RNA extraction with protocol modifications on the lysis step (Method BL) was compared on 23 matching FFPE cardiac tissue specimens (n = 92).
View Article and Find Full Text PDFBackground: Neonatal herpes simplex virus (HSV) infection is life-threatening, with a mortality of up to 70-80% when disseminated, often due to vague symptoms and delayed treatment. Neonatal screening using dried blood spot (DBS) samples is among the most impactful preventative health measures ever implemented, but screening for HSV has not been investigated.
Methods: We investigated high throughput multiplexed proteomics on DBS samples collected on days 2-3 of life from a nationwide cohort of neonates with HSV infection (n = 53) and matched controls.
Mandibular advancement devices are an effective treatment option for obstructive sleep apnea. While their efficacy depends on the degree of mandibular protrusion, other contributing factors influencing the optimal outcome are not fully understood. This magnetic resonance imaging-based pilot study aimed at investigating whether there are promising planimetric parameters that may be related to the optimal therapeutic position.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to improve emergency trauma care by implementing the "Double 90 rule," which activates additional medical teams for critically injured patients with low blood pressure.
- Researchers found that implementing this rule significantly reduced the time to key interventions, such as CT scans and hemorrhage control procedures, over a three-year period.
- Although there were improvements in care, mortality rates remained similar between patients treated before and after the rule was put into place.
Article Synopsis
- Cardiac pressure overload can lead to problems for patients with congenital heart defects, and using stem cell-derived heart cells might help improve heart function alongside surgery.
- Researchers successfully implanted human stem cell-derived heart cells into the hearts of rhesus macaques facing induced pressure overload, achieving good integration with the existing heart tissue.
- Although some monkeys experienced episodes of ventricular tachycardia after the cell transplant, these events generally resolved within a few weeks, indicating a need for monitoring but also promising potential for this treatment approach.