Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction.

Ribosomes translate the genetic code into proteins. Recent technical advances have facilitated in situ structural analyses of ribosome functional states inside eukaryotic cells and the minimal bacterium Mycoplasma. However, such analyses of Gram-negative bacteria are lacking, despite their ribosomes being major antimicrobial drug targets.

A molecular device for the redox quality control of GroEL/ES substrates.

Hsp60 chaperonins and their Hsp10 cofactors assist protein folding in all living cells, constituting the paradigmatic example of molecular chaperones. Despite extensive investigations of their structure and mechanism, crucial questions regarding how these chaperonins promote folding remain unsolved. Here, we report that the bacterial Hsp60 chaperonin GroEL forms a stable, functionally relevant complex with the chaperedoxin CnoX, a protein combining a chaperone and a redox function.

Assessment of vulnerability to water shortage in semi-arid river basins: The value of demand reduction and storage capacity.

Interest in securing reliable water supplies has increased due to climate change and rapid population growth. This challenge is significant in growing areas with limited water supplies. To meet water demands, water managers are considering new storage infrastructure to increase the reliability of water supplies while also identifying opportunities to reduce water use per person.

Changing climate, changing minds? The effects of natural disasters on public perceptions of climate change.

The frequency and intensity of natural disasters such as hurricanes, wildfires, and floods are predicted to change as greenhouse gas concentrations increase. These disasters may represent sources of information for individuals as they update their beliefs related to climate change. Using a dataset that includes climate beliefs of respondents, we examine the effect of natural disasters on climate change beliefs and find that hurricanes significantly increase the probability that survey respondents from a given county believe that climate change is occurring and that it is human caused.