The Cerebrospinal Fluid Free-Glycans Hex and HexNAcHexNeu5Ac as Potential Biomarkers of Alzheimer's Disease.

Alzheimer's disease (AD) is the most common neurodegenerative disorder, affecting a growing number of elderly people. In order to improve the early and differential diagnosis of AD, better biomarkers are needed. Glycosylation is a protein post-translational modification that is modulated in the course of many diseases, including neurodegeneration.

Comparison of diagnostic routines for suspected Alzheimer's disease patients in US-American and German primary care.

Thorough diagnostics are a prerequisite for the optimal treatment of Alzheimer's disease (AD). Biomarker-based diagnostics are standard in academia, data on practitioners' diagnostic workups is scarce. Surveys in German and US healthcare providers (HCP) were conducted regarding diagnostics in presumed AD patients.

Influence of preanalytical and analytical factors on the quantification of six regulatory serum proteins.

We analyzed differences in protein concentrations in human blood serum depending on the tube material and the immunoassay platform used. Blood samples from study participants were collected in glass and polypropylene tubes (n = 292). Serum concentrations of six proteins (BDNF, IGF-1, VEGF-A, TGF-β1, MCP-1 and IL-18) were assessed by using ELISAs (all biomarkers), as well as a novel fully automated immunoassay platform (all but IGF-1, n = 211).

Long-term stability and age-dependence of six regulatory serum proteins.

The development of biomarker-based diagnostic procedures often relies on samples stored for several years. We aimed to investigate the influence of storage time and patient age on six neuroregulatory and immunoregulatory serum biomarkers. We quantified six biomarkers in serum from 151 individuals using ELISA.

Presenilin 1 Gene Mutation (M139V) in a German Family with Early-Onset Alzheimer's Disease: A Case Report.

Objective: This study describes a 44-year-old German male with early-onset Alzheimer's disease as a result of a M139V presenilin 1 mutation. The patient has at least seven affected family members, spanning at least four generations.

Method: We performed a complete demographic, genetic, neuropsychological, neuropsychiatric, neuroradiological, and neuropathological characterizations of this patient.