Immune monitoring of blood in heart transplant recipients: application of flow cytometry.

In this study, we review the utility of peripheral blood lymphocyte evaluation using commercially available monoclonal antibodies in the diagnosis of rejection or infection in 27 patients undergoing allograft heart transplantation. Double-color staining methods employing fluorescein isothiocyanate (FITC) and phycoerythrin (PE) allowed identification of a variety of lymphocyte subsets and determination of activation or proliferation of lymphocytes. All patients also underwent regular endomyocardial biopsy procedures.

Identification of a novel human B cell activation antigen involved in B cell growth factor-dependent proliferation.

The B6.4 mAb we present here identifies a novel activation Ag of B cell lineage. The B6.

Development and characterization of a human B cell line that responds to B cell growth factor but not interleukin 2.

The human lymphoblastoid cell line we present here proliferated in response to a 14,000 m.w. B cell growth factor (BCGF), and not to interleukin 2 (IL 2).

Endonuclease specific for apurinic/apyrimidinic sites from human liver.

The endonuclease (AP endonuclease) specific for apurinic/apyrimidinic sites (AP sites) has been purified and characterized from normal human autopsy liver. Three chromatographically distinct species of AP endonuclease were resolved by phosphocellulose chromatography. The species had molecular weights ranging from 24,000 to 31,000.