CircTHADA regulates endothelial cell pyroptosis in diabetic retinopathy through miR-494-3p/CASP1/GSDMD-N/IL-1β pathway.

Our study aimed to elucidate the mechanism by which circTHADA competitively adsorbs miR-494-3p to regulate CASP1-mediated endothelial cell (EC) pyroptosis in diabetic retinopathy (DR). To be specific, we used high glucose (HG)-induced human retinal microvascular endothelial cells (HRMECs) as DR cell models and streptozotocin (STZ)-treated mice as DR mouse models. The expression levels of circTHADA, miR-494-3p, CASP1, NLRP3, GSDMD-N and IL-1β were detected and flow cytrometry was applied to measure cell pyroptosis rate and dual luciferase reporter assays were utilized to determine the direct binding sites.

Nucleus pulposus cell-mimicking nanoparticles for cell-specific HIF1A editing to modulate SASP-mediated disc inflammation via autophagy activation.

Traditional methods of plasmid delivery, including viral vectors, lipofection, and electroporation, are widely used for gene editing but have limitations, such as cellular toxicity, limited transfection efficiency in primary cells, and nonspecific side effects. Here, we report the development of nucleus pulposus cell (NPC)-mimicking nanoparticles (HIF1A@NNP) with an NPC membrane as the shell and pcDNA3.1-rHIF1A encapsulated in the core via extrusion.

Suture Anchor and Reverse Suture Anchor show Superior Biomechanical Efficacy to Transtibial Pull-out Repair for Posterior Medial Meniscus Root Tears in Porcine Model.

Purpose: To investigate the biomechanical efficacy of the reverse suture anchor (RSA) technique compared with the transtibial pull-out (TP) and suture anchor (SA) techniques using in vitro porcine knee models of PMMRTs.

Methods: 32 fresh frozen porcine tibiae with medial meniscus intact were randomly assigned to four groups (eight specimens each). A standardized posterior medial meniscus root tear (PMMRT) was established in 24 specimens.

Knowledge mapping and research trends of cathelicidin peptide LL-37 from 1995 to 2024: a bibliometric study.

Background: To conduct a comprehensive bibliometric analysis of LL-37, summarize its development trends and patterns, and identify emerging research hotspots.

Methods: Bibliometric and Knowledge Graph Analysis of Literature Data Related to LL-37 in the WOSCC Database Using Citespace and Vosviewer.

Results: A total of 2,814 articles were analyzed, revealing a steady increase in recent publications.

Piezo1 specific deletion in macrophage protects the progression of chronic inflammatory bowel disease in mice.

Background &aims: Piezo1, a recently identified mechanically-activated nonselective cation channel protein, demonstrates sensitivity to various mechanical stimuli, such as matrix stiffness and shear stress. While accumulating evidence implicates Piezo1 channels in numerous physiological and pathophysiological processes, its involvement in dextran sulfate sodium (DSS)-induced acute and chronic inflammatory bowel disease (IBD) remains incompletely understood. This study aimed to investigate the effect of Piezo1 channels in macrophage polarization and its associated functions in IBD.