Estimating the impact of 2D urban landscape patterns on extreme precipitation based on non-stationary models in the Guangdong-Hong Kong-Macao Greater Bay Area, China.

Rapid urbanization has significantly altered surface landscape configurations, leading to complex urban climates. While much attention has been focused on impervious surfaces' impact on extreme precipitation, a critical gap remains in understanding how various 2D urban landscape components influence extreme precipitation across different durations. Through an analysis of the non-stationarity and spatiotemporal variations in extreme precipitation across the Guangdong-Hong Kong-Macao Greater Bay Area (GBA) from 1990 to 2020, we constructed the non-stationary Generalized Additive Models for Location Scale and Shape (GAMLSS) model by introducing six urban landscape structural metrics as explanatory variables for each of the 27 meteorological stations in the GBA.

Multiplexed inhibition of immunosuppressive genes with Cas13d for combinatorial cancer immunotherapy.

The complex nature of the immunosuppressive tumor microenvironment (TME) requires multi-agent combinations for optimal immunotherapy. Here we describe multiplex universal combinatorial immunotherapy via gene silencing (MUCIG), which uses CRISPR-Cas13d to silence multiple endogenous immunosuppressive genes in the TME, promoting TME remodeling and enhancing antitumor immunity. MUCIG vectors targeting four genes delivered by adeno-associated virus (AAV) (Cd274/Pdl1, Lgals9/Galectin9, Lgals3/Galectin3 and Cd47; AAV-Cas13d-PGGC) demonstrate significant antitumor efficacy across multiple syngeneic tumor models, remodeling the TME by increasing CD8 T-cell infiltration while reducing neutrophils.

TRIM28 is an essential regulator of three-dimensional chromatin state underpinning CD8 T cell activation.

T cell activation is accompanied by extensive changes in epigenome. However, the high-ordered chromatin organization underpinning CD8 T cell activation is not fully known. Here, we show extensive changes in the three-dimensional genome during CD8 T cell activation, associated with changes in gene transcription.

Strategically constructed hybrid bulk-heterojunction for efficient flexible self-powered infrared photodetectors with a carbon electrode.

The construction of an admirable hybrid bulk-heterojunction (HBH) can benefit the performance of optoelectronic devices through efficient charge separation and transportation. However, the present HBH structure still suffers from complicated layer-by-layer ligand exchanges during device fabrication. In this work, we apply a liquid phase exchange strategy in mixed colloidal hybrids composed of quantum dots (QDs) and nanotetrapods (NTs) and construct low-cost flexible self-powered infrared photodetectors with a carbon electrode.