Chemokine receptor-derived peptides as multi-target drug leads for the treatment of inflammatory diseases.

The rationale for multi-target drugs has been strengthened both on theoretical and empirical grounds. Serious diseases that are intractable to treatment were found to have multiple pathogenic factors and examples of successful drugs were shown to affect multiple disease targets. The salient features of multiple-target drugs, low target affinity and rapid binding kinetics, have been responsible for their late discovery and slow development.

Physiological immunity or pathological autoimmunity--a question of balance.

Chemokines (CKs) are chemo-attractants that mobilize and activate leukocytes of the immune system. CKs and their receptors have become targets for drug discovery and development on the basis of correlations between their expression profiles and autoimmune diseases. Essential for both physiological immunity and pathological autoimmunity, these immune messengers and regulators have proven to be tantalizing drug targets.

Specific gene expression in pancreatic beta-cells: cloning and characterization of differentially expressed genes.

Identification and characterization of genes expressed preferentially in pancreatic beta-cells will clarify the mechanisms involved in the specialized properties of these cells, as well as providing new markers of the development of type 1 diabetes. Despite major efforts, relatively few beta-cell-specific genes have been characterized. We applied representational difference analysis to identify genes expressed selectively in the pancreatic beta-cell line betaTC1 compared with the pancreatic alpha-cell line alphaTC1 and isolated 26 clones expressed at higher levels in the beta-cells than in the alpha-cells.