Publications by authors named "C E Koering"
Background: Cell differentiation requires the integration of two opposite processes, a stabilizing cellular memory, especially at the transcriptional scale, and a burst of gene expression variability which follows the differentiation induction. Therefore, the actual capacity of a cell to undergo phenotypic change during a differentiation process relies upon a modification in this balance which favors change-inducing gene expression variability. However, there are no experimental data providing insight on how fast the transcriptomes of identical cells would diverge on the scale of the very first two cell divisions during the differentiation process.
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- * In a study with two cohorts, skipping of TET2 exon 2 (TET2E2S) was independent of age and cytogenetics and associated with a significantly lower risk of relapse and improved survival, especially in younger patients with cytogenetically normal AML.
- * TET2E2S was the only prognostic factor for overall survival and positively influenced survival across various genetic risk categories, suggesting that monitoring TET2 exon 2 splicing could enhance patient risk assessments in AML treatments.
Azacitidine inhibits DNA methyltransferases, including DNMT1, and is currently the standard of care for patients with higher-risk myelodysplastic syndrome (HRMDS) or low blast count acute myeloid leukemia (AML). The expression of 754 miRNAs was compared in azacitidine-resistant and azacitidine-sensitive myelodysplastic syndrome cells. We investigated the role of differentially expressed miRNAs on DNMT1 expression and azacitidine resistance We next evaluated anti-DNMT1 miRNA expression in pretreatment bone marrow samples derived from 75 patients treated with azacitidine for HRMDS or AML.
View Article and Find Full Text PDFReplicative senescence is a hallmark of chronic liver diseases including chronic hepatitis B virus (HBV) infection, whereas HBV-encoded oncoproteins HBx and preS2 have been found to overcome senescence. HBx possesses a C-terminal truncation mainly in hepatocellular carcinomas but also in noncancerous liver tissues. Here, by cell counting, BrdU incorporation, MTT proliferation assay, cell cycle analysis, SA-βgal staining and Western blotting in primary and malignant cells, we investigated the effect of HBx C-terminal mutants on cellular senescence.
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- The study investigates how mutations in spliceosome genes, oncogene expression, and drug resistance in Acute Myeloid Leukemia (AML) affect exon expression.
- Exon-array analysis and exon-specific PCR were used to analyze exon expression in AML cell lines and patient samples, finding over 70% validation of identified exon events.
- Results highlighted significant differences in exon events between chemosensitive and chemoresistant AML, revealing new potential pathways for targeting drug resistance linked to DEK and WT1 oncogenes.