Prolactin levels during the menstrual cycle.

The levels of prolactin, FSH, LH, oestradiol and progesterone were measured daily during fourteen ovulatory cycles. The behaviour of FSH, LH, oestradiol and progesterone was classical. Non-systematic changes occurred in prolactin levels during the course of the menstrual cycle with the highest level being either during the ovulatory period or during the luteal phase.

[Dosage of prolactin in normal and pathological subjects (author's transl)].

Homologous radioimmunoassay of human prolactin was performed and used to investigate the secretion of this pituitary hormone under normal and pathological conditions. The following data were developed in this paper: --During the menstrual cycle, prolactin levels are more elevated during ovulatory and luteal phases than during follicular phase; --During pregnancy, prolactin levels progressively increase to reach their maximum at the end of gestation; --During lactation, prolactin levels increase for the two days following delivery, then progressively decrease; --Bromocryptine oral administration drastically reduces prolactin levels within the 24 hours as it inhibits the prolactin release normally induced by suckling. Assays of prolactin is useful to define the role of this hormone in the genesis of the galactorrhea.

[Applications of the extraction and of the radioimmunoassay of LH-RH in human urine].

The existence of endogenous LH RH like immunoreactivity is shown in human urines after appropriate extraction, bu the radioimmunoassay of LH RH. In normaly cycling and menopausal women the quantities of endogenous hormone found in urines are greater after acid extraction than those found after extraction at pH 7. Furthermore, the increase observed by extraction in acidified methanol is directly correlated and proportional to the quantity of hormone assayable by extraction at pH 7.

[Extraction of LHRH in human urine: study of the extraction of labelled synthetic hormone].

A method of extraction of synthetic LHRH is studied in human urines, using porous glass (Spherosil) and methanol. In the defined conditions the yield is greeter than 80%. It appears that the method is reproducible.