A new C-20 polyacetylene from the sponge Callyspongia pseudoreticulata.

The Indonesian marine sponge Callyspongia pseudoreticulata was found to contain (3S,18S,4E,16E)-eicosa-1,19-diyne-3,18-diol-4,16-diene (1), the structure of which was determined by detailed spectroscopic analysis. Its absolute configuration was established using the modified Mosher's method after esterification of the secondary alcohols with Mosher's reagent.

Primary cultures from the marine sponge Xestospongia muta (Petrosiidae, Haplosclerida).

In the context of the investigations on the origin and in vitro production of bioactive compounds, primary cultures were developed from ectosomal and choanosomal cell suspensions from the sponge Xestospongia muta. Dissociated cells aggregated and reorganized into a striking reticulated network of cells, typical for X. muta.

5-hydroxytryptamine-derived alkaloids from two marine sponges of the genus Hyrtios.

Indonesian specimens of the marine sponges Hyrtios erectus and H. reticulatus were found to contain 5-hydroxytryptamine-derived alkaloids. Their structures were determined on the basis of their spectral properties.

Cellular location of (2R, 3R, 7Z)-2-aminotetradec-7-ene-1, 3-diol, a potent antimicrobial metabolite produced by the Caribbean sponge Haliclona vansoesti.

The Caribbean sponge Haliclona vansoesti has been found to contain large amounts of a new sphingosine derivative, (2R, 3R, 7Z)-2-aminotetradec-7-ene-1, 3-diol (compound 1). To determine the localization of this compound within the organism, cell distribution and quantitative determination of the aminodiol content of cell fractions obtained by differential centrifugation have been performed. Results show that choanocytes and archaeocytes are the major sponge cell types and that H.

(2R,3R,7Z)-2-Aminotetradec-7-ene-1,3-diol, a new amino alcohol from the caribbean sponge Haliclona vansoesti.

The Caribbean marine sponge Haliclona vansoesti was found to contain a high amount (5% dry wt) of a new analogue of 4-sphingenine, (2R,3R, 7Z)-2-aminotetradec-7-ene-1,3-diol (1). Its structure was determined by detailed spectroscopic analysis. The relative configuration was deduced from the NMR data of the corresponding acetonide 5, while the absolute configuration was secured via protection of the primary alcohol and amino groups, and esterification of the secondary alcohol with Mosher's reagent.