Nattokinase as a novel food-grade enzyme for long term cell subculture.

Cellular agriculture and regenerative medicine strive to replace animal-derived products in their processes as much as possible. In these efforts dissociation enzymes required for cell detachment from their substrate are often overlooked. Cell detachment is an essential part of subculturing and to date it is achieved using either animal pancreas-derived trypsin or expensive recombinant enzymes.

Review: Livestock cell types with myogenic differentiation potential: Considerations for the development of cultured meat.

With the current environmental impact of large-scale animal production and societal concerns about the welfare of farm animals, researchers are questioning whether we can cultivate animal cells for the purpose of food production. This review focuses on a pivotal aspect of the cellular agriculture domain: cells. We summarised information on the various cell types from farm animals currently used for the development of cultured meat, including mesenchymal stromal cells, myoblasts, and pluripotent stem cells.