A nucleoside signal generated by a fungal endophyte regulates host cell death and promotes root colonization.

The intracellular colonization of plant roots by the beneficial fungal endophyte Serendipita indica follows a biphasic strategy, including a host cell death phase that enables successful colonization of Arabidopsis thaliana roots. How host cell death is initiated and controlled is largely unknown. Here, we show that two fungal enzymes, the ecto-5'-nucleotidase SiE5NT and the nuclease SiNucA, act synergistically in the apoplast at the onset of cell death to produce deoxyadenosine (dAdo).

An Improved Bio-Physical Parameterization for Ocean Radiant Heating in Conditions of Near-Surface Stratification.

Solar heating of the upper ocean is a primary energy input to the ocean-atmosphere system, and the vertical heating profile is modified by the concentration of phytoplankton in the water, with consequences for sea surface temperature and upper ocean dynamics. Despite the development of increasingly complex modeling approaches for radiative transfer in the atmosphere and upper ocean, the simple parameterizations of radiant heating used in most ocean models can be significantly improved in cases of near-surface stratification. There remains a need for a parameterization that is accurate in the upper meters and contains an explicitly spectral dependence on the concentration of biogenic material, while maintaining the computational simplicity of the parameterizations currently in use.

Enhancing patient activation: a controlled implementation study of an interprofessional evidence-based counseling program for complementary and integrative healthcare in cancer patients ('CCC-Integrativ').

Complementary and integrative healthcare (CIH) is increasingly recognized as a valuable approach to empowering and activating cancer patients. Studies have shown that higher patient activation is positively associated with improved health outcomes and reduced healthcare costs. The CCC-Integrativ study aimed to assess the implementation of an evidence-based counseling service on CIH at four Comprehensive Cancer Centers (CCC) in Germany.

Uracil phosphoribosyltransferase is required to establish a functional cytochrome bf complex.

Arabidopsis uracil phosphoribosyltransferase (UPP) is an essential enzyme and plants lacking this enzyme are strongly compromised in chloroplast function. Our analysis of UPP amiRNA mutants has confirmed that this vital function is crucial to establish a fully functional photosynthesis as the RIESKE iron sulfur protein (PetC) is almost absent, leading to a block in photosynthetic electron transport. Interestingly, this function appears to be unrelated to nucleotide homeostasis since nucleotide levels were not altered in the studied mutants.

Nucleotides and nucleotide derivatives as signal molecules in plants.

In reaction to a stimulus, signaling molecules are made, generate a response, and are then degraded. Nucleotides are classically associated with central metabolism and nucleic acid biosynthesis, but there are a number of nucleotides and nucleotide derivatives in plants to which this simple definition of a signaling molecule applies in whole or at least in part. These include cytokinins and chloroplast guanosine tetraposphate (ppGpp), as well as extracellular canonical nucleotides such as extracellular ATP (eATP) and NAD+ (eNAD+).