Postbiotic-biosynthesized silver nanoparticles anchored on covalent organic frameworks integrated into carboxymethyl chitosan-based film for enhancing antibacterial packaging.

Food packaging plays a vital role in guaranteeing the quality and safety of fresh products during the storage and distribution. Carboxymethyl chitosan (CMCS) is identified as a promising polymer for food packaging film owing to its film-forming ability, non-toxicity, and biodegradability. Nevertheless, the practical applications of pure CMCS film usually suffer from some limits owing to its poor antibacterial effect and mechanical strength.

CRISPR/Cas System Meets CLICK-17 DNAzyme: A Click Chemistry-Based Fluorescence Biosensing Platform Designed for Highly Sensitive Detection of .

is one of the most dangerous and contagious foodborne pathogens, posing a significant threat to public health and food safety. In this study, we developed a click chemistry-based fluorescence biosensing platform for highly sensitive detection of () by integrating the -cleavage activity of CRISPR/Cas12a with the CLICK17-mediated copper(II)-dependent azide-alkyne cycloaddition (Cu(II)AAC) click reaction. Herein, CLICK-17 can provide binding sites for Cu ions and high redox stability for one or much catalytically vital Cu within its active sites, which facilitate the click reaction.

Donkey Milk Supplementation Alleviates Renal Fibrosis of Chronic Kidney Disease by Enhancing Anti-inflammatory Ability.

Donkey milk, rich in essential nutrients, not only can meet the nutritional needs of patients with chronic kidney disease (CKD) accodding to the National Kidney Foundation's Kidney Disease Outcomes Quality Initiative (KDOQI) Clinical Practice Guideline for Nutrition in CKD, but also has strong anti-inflammatory ability. Inflammation is the main driving force of renal fibrosis, directly determining the renal function of CKD. However, the impact of donkey milk on CKD and the underlying mechanism remains unclear.

Daily glucose variability is associated with intrahepatic fat content, β cell sensitivity, and biomarkers of glycolysis in youth with obesity.

Objective: Pediatric obesity is associated with insulin resistance, which, in turn, impacts glucose and lipid metabolism. This study sought to assess how glucose variability relates to intrahepatic fat content, β cell insulin sensitivity, and glycolysis in youth with obesity.

Methods: A total of 27 youth with obesity (11 girls, BMI percentile, median [25th-75th percentiles]: 99.