The function of BCL11B in base excision repair contributes to its dual role as an oncogene and a haplo-insufficient tumor suppressor gene.

Genetic studies in mice and human cancers established BCL11B as a haploinsufficient tumor suppressor gene. Paradoxically, BCL11B is overexpressed in some human cancers where its knockdown is synthetic lethal. We identified the BCL11B protein in a proximity-dependent biotinylation screen performed with the DNA glycosylase NTHL1.

The DNA repair function of BCL11A suppresses senescence and promotes continued proliferation of triple-negative breast cancer cells.

We identified the BCL11A protein in a proximity-dependent biotinylation screen performed with the DNA glycosylase NTHL1. , DNA repair assays demonstrate that both BCL11A and a small recombinant BCL11A protein that is devoid of DNA binding and transcription regulatory domains can stimulate the enzymatic activities of two base excision repair enzymes: NTHL1 and DNA Pol β. Increased DNA repair efficiency, in particular of the base excision repair pathway, is essential for many cancer cells to proliferate in the presence of elevated reactive oxygen species (ROS) produced by cancer-associated metabolic changes.

Base excision repair accessory factors in senescence avoidance and resistance to treatments.

Cancer cells, in which the RAS and PI3K pathways are activated, produce high levels of reactive oxygen species (ROS), which cause oxidative DNA damage and ultimately cellular senescence. This process has been documented in tissue culture, mouse models, and human pre-cancerous lesions. In this context, cellular senescence functions as a tumour suppressor mechanism.

Factors behind the Relinquishment of Dogs and Cats by their Guardians in Portugal.

In Portugal, the relinquishment of dogs and cats is a major problem. Thus, the authors developed a questionnaire to understand the factors influencing the relinquishment of companion animals in Portugal. With a view to analyze the factors behind relinquishment by companion animal guardians, the authors analyzed surveys completed by 72 participants, divided into two groups: REL (relinquisher, n = 36), i.