The vertical distribution of tetA and intI1 in a deep lake is rather due to sedimentation than to resuspension.

Lakes are exposed to anthropogenic pollution including the release of allochthonous bacteria into their waters. Antibiotic resistance genes (ARGs) stabilize in bacterial communities of temperate lakes, and these environments act as long-term reservoirs of ARGs. Still, it is not clear if the stabilization of the ARGs is caused by a periodical introduction, or by other factors regulated by dynamics within the water column.

Screening of a Library of Oligosaccharides Targeting Lectin LecB of and Synthesis of High Affinity Oligoglycoclusters.

The Gram negative bacterium (PA) is an opportunistic bacterium that causes severe and chronic infection of immune-depressed patients. It has the ability to form a biofilm that gives a selective advantage to the bacteria with respect to antibiotherapy and host defenses. Herein, we have focused on the tetrameric soluble lectin which is involved in bacterium adherence to host cells, biofilm formation, and cytotoxicity.

Synthesis of α-galactosyl epitopes by metabolically engineered Escherichia coli.

The α-Gal epitope is a carbohydrate structure, Galα-3Galβ-4GlcNAc-R, expressed on glycoconjuguates in many mammals, but not in humans. Species that do not express this epitope have present in their serum large amounts of natural anti-Gal antibodies, which contribute to organ hyperacute rejection during xenotransplantation. We first describe the efficient conversion of lactose into isoglobotriaose (Galα-3Galβ-4Glc) using high cell density cultures of a genetically engineered Escherichia coli strain expressing the bovine gene for α-1,3-galactosyltransferase.

Influence of wetting conditions on drainage in porous media: a microfluidic study.

This paper presents some experimental results on two-phase flows in model two-dimensional (2D) porous media with different wetting properties. Standard microfluidic techniques are used to fabricate the 2D micromodels that consist of a network of straight microchannels having heterogeneous sizes. The invasion mechanism is analyzed quantitatively for partial and total wetting conditions, and for various stable viscosity ratios and capillary pressure heterogeneity.

Drainage in two-dimensional porous media: from capillary fingering to viscous flow.

This paper reports some experimental results on two-phase flows in model two-dimensional porous media. Standard microfluidic techniques are used to fabricate networks of straight microchannels and to control the throat size distribution. We analyze both the invasion mechanism of the medium by a nonwetting fluid and the drainage after the percolation for capillary numbers lying between 10(-7) and 10(-2).