Role of the Trichoderma harzianum endochitinase gene, ech42, in mycoparasitism.

The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants.

Characterization and gene expression of nodulin Npv30 from common bean.

We previously reported that transcripts for a 30-kD nodulin (Npv30) are very abundant in the nodule. In this paper we describe the isolation and characterization of Npv30 cDNA and genomic clones. Npv30 has the following characteristic features: (a) a putative signal sequence at the deduced amino-terminal region, (b) a proline-rich stretch at the carboxy terminus, and (c) a characteristic domain of four cysteines that resemble metal-binding sites.

The expression of a chimeric Phaseolus vulgaris nodulin 30-GUS gene is restricted to the rhizobially infected cells in transgenic Lotus corniculatus nodules.

In Phaseolus vulgaris there is a nodulin family, Npv30, of ca. 30 kDa, as detected in an in vitro translation assay [2]. We isolated a gene (npv30-1) for one of the members of this family.

Characterization of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism.

A gene (ech-42; previously named ThEn-42) coding for one of the endochitinases produced by the biocontrol agent Trichoderma harzianum IMI206040 was cloned and characterized. Expression of the cDNA clone in Escherichia coli resulted in bacteria with chitinase activity. This chitinase has been shown to have lytic activity on Botrytis cinerea cell walls in vitro.