Publications by authors named "C CASTANEIRA"

Members of the Equus genus exhibit a fascinating capacity for hybridization, giving rise to healthy offspring. Mules, resulting from the mating of a mare with a jack, represent the most prevalent equid hybrid, serving diverse roles in our society. While in vitro embryo production, particularly through Intracytoplasmic Sperm Injection (ICSI), has rapidly gained significance in domestic horses, the in vitro production in other equids remains largely unexplored.

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In horses, prostaglandin E (PGE) is produced by embryos around Day 5 post-ovulation; PGE functions directly at the oviduct promoting embryo transport into the uterus. Non-surgical collection of horse embryos for cryopreservation is recommended at Day 6.5-7 post-ovulation.

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Normal mammalian early embryonic development involves apoptosis of blastomeres as a remodeling process during differentiation, starting at the blastocyst stage. Genomic DNA has been recently detected in the blastocele fluid of human embryos and has been amplified by real-time polymerase chain reaction (PCR) to diagnose the sex of in vitro-produced human embryos. This new approach varies from conventional preimplantation genetic diagnosis in that no cells are extracted from the embryo and only the blastocele fluid is aspirated and used as a DNA sample for diagnosis.

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Article Synopsis
  • This study evaluated the use of air-dried sperm for creating equine embryos and examined the effects of sperm extract activation and in vivo culture on embryo production.
  • Results showed that using sperm extract for activation increased the cleavage rate of embryos, but there were no significant differences in overall embryo development between activation methods or storage times.
  • It was concluded that air-dried sperm can produce equine embryos after several weeks, and while sperm extract improves initial cleavage, in vivo culture methods lead to the successful development of embryos at later stages.
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