Publications by authors named "C C Deelen"
Article Synopsis
- The study investigates the relationship between the post-thaw morphology of cryopreserved equine IVP blastocysts and the likelihood of achieving pregnancy after transfer.
- The findings show that while the biopsy procedure before freezing does not negatively impact pregnancy outcomes, blastocysts with over 10% herniated cells post-thaw are less likely to result in pregnancy.
- Factors such as the interval between ICSI and blastocyst confirmation, and the specific stallion and mare involved, significantly influence pregnancy rates, with later developing embryos showing lower success rates.
The cryotolerance of equine blastocysts larger than 300 μm can be improved by aspirating blastocoele fluid prior to vitrification; however, it is not known whether blastocoele aspiration also enables successful slow-freezing. The aim of this study was therefore to determine whether slow-freezing of expanded equine embryos following blastocoele collapse was more or less damaging than vitrification. Grade 1 blastocysts recovered on day 7 or 8 after ovulation were measured (>300-550 μm, n = 14 and > 550 μm, n = 19) and blastocoele fluid was aspirated prior to slow-freezing in 10% glycerol (n = 14), or vitrification (n = 13) in 16.
View Article and Find Full Text PDFTranscriptome studies of Illumina RNA-Seq datasets of different Arabidopsis thaliana natural accessions and T-DNA mutants revealed the presence of two virus-like RNA sequences which showed the typical two-segmented genome characteristics of a comovirus. This comovirus did not induce any visible symptoms in infected A. thaliana plants cultivated under standard laboratory conditions.
View Article and Find Full Text PDFAneuploidy of meiotic origin is a major contributor to age-related subfertility and an increased risk of miscarriage in women. Although age-related aneuploidy has been studied in rodents, the mare may be a more appropriate animal model to study reproductive aging. Similar to women, aged mares show reduced fertility and an increased incidence of early pregnancy loss; however, it is not known whether aging predisposes to aneuploidy in equine oocytes.
View Article and Find Full Text PDFBackground: In vitro embryo production (IVEP) is increasingly popular but data assessing the outcome of transferred embryos are scarce.
Objectives: To determine the likelihood of pregnancy and embryonic loss after transfer of frozen-thawed IVP embryos and identify factors influencing success.
Study Design: Retrospective clinical study.