Monodisperse Chemical Oligophosphorylation of Peptides via Protected Oligophosphorimidazolide Reagents.

Protein poly- and oligophosphorylation are recently discovered post-translational modifications that remain poorly characterized due to (1) the difficulty of extracting endogenously polyphosphorylated species without degradation and (2) the absence of synthetic and analytical tools to prepare and characterize poly- and oligophosphorylated species in biochemical contexts. Herein, we report a methodology for the selective oligophosphorylation of peptides with monodisperse phosphate chain lengths (P=3-6). A library of oligophosphorimidazolide (oligoP-imidazolide) reagents featuring benzyl and o-nitrophenylethyl protecting groups was synthesized in moderate-to-good yields (65-93 %).

The European Nucleotide Archive in 2024.

The European Nucleotide Archive (ENA, https://www.ebi.ac.

Effects of snake fungal disease (ophidiomycosis) on the skin microbiome across two major experimental scales.

Emerging infectious diseases are increasingly recognized as a significant threat to global biodiversity conservation. Elucidating the relationship between pathogens and the host microbiome could lead to novel approaches for mitigating disease impacts. Pathogens can alter the host microbiome by inducing dysbiosis, an ecological state characterized by a reduction in bacterial alpha diversity, an increase in pathobionts, or a shift in beta diversity.

Arene extrusion as an approach to reductive elimination at boron: implication of carbene-ligated haloborylene as a transient reactive intermediate.

Herein, we report boron-centered arene extrusion reactions to afford putative cyclic(alkyl)(amino) carbene (CAAC)-ligated chloroborylene and bromoborylene intermediates. The borylene precursors, chloro-boranorbornadiene (ClB(CMe), 2) and bromo-boranorbornadiene (BrB(CMe), 2) were synthesized through the reaction of the corresponding 1-halo-2,3,4,5-tetramethylborole dimer (XBCMe) (X = Cl, 1; X = Br, 1) with 2-butyne. Treatment of 2 with CAACs resulted in the release of di-coordinate chloro-borylene (CAAC)BCl from hexamethylbenzene (CMe) at room temperature.